Fin gRNA punches

Dissect out the clone using a fresh razor to avoid contamination.

 

Prepare a library for the punch(es), following this gRNA Punch MiSeq Library guide.

Submit library to CGS for spike in.

Create a plaintext file (not .doc or.docx) with all the library names in it, one name per line.

Run a gRNA identifier with the command:

perl multifile.miseq_gRNA_sort.txt   <your plaintext list of files>   24k_grnas_unix

 

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