Dissect out the clone using a fresh razor to avoid contamination.
Prepare a library for the punch(es), following this gRNA Punch MiSeq Library guide.
Submit library to CGS for spike in.
Create a plaintext file (not .doc or.docx) with all the library names in it, one name per line.
Run a gRNA identifier with the command:
perl multifile.miseq_gRNA_sort.txt <your plaintext list of files> 24k_grnas_unix
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