s.w.e Schedl Lab

Washington University Genetics

DAPI Staining

Protocol by Ross Francis Modified by Sudhir Nayak To visualize DNA, incubate fixed samples with 100 ng/ml 4′,6′-diamidino-2-phenylindole hydrochloride (DAPI) in PBT. The staining time depends on the type of sample and varies from 5-30 min. For example, 5 min is long enough for dissected gonad arms but longer incubation (20-30 min) is required for […]

  • Tim Schedl, Ph.D
    Department of Genetics
    Campus Box #8232
    Washington University School of Medicine
    4566 Scott Ave.
    St. Louis, MO 63110


    Email: ts@genetics.wustl.edu
    PHONE: (314)362-6164 [lab]
    FAX: (314)362-7855