s.w.e Schedl Lab

Washington University Genetics

EdU labeling and staining

EdU labeling and fixation (Modified by Schedl lab from protocol provided by Sarah Crittenden) To label on plates: (from Ito and McGhee, 1987) 1. Grow an overnight of MG1693 (thy-, from E. coli stock center) 2. To 100 ml of M9, add: ~2 ml ON MG1693 5 ml 20% glucoseĀ  (0.4%-1% – usually 1%) 100 […]

  • Tim Schedl, Ph.D
    Department of Genetics
    Campus Box #8232
    Washington University School of Medicine
    4566 Scott Ave.
    St. Louis, MO 63110

    Contact

    Email: ts@genetics.wustl.edu
    PHONE: (314)362-6164 [lab]
    FAX: (314)362-7855