Protocol for Double-Stranded RNA Synthesis By Sudhir Nayak 1.  Make both sense and antisense RNA strands separately (we use Ambion’s RNA Transcription Kit). 2.  After the synthesis reactions, DNAse (10 units) the samples for one 30-60 min at 37 C. 3.  Mix both antisense and sense strands together in the same tube. 4.  Heat the […]