s.w.e Schedl Lab

Washington University Genetics

dsRNA (double-stranded RNA) for injection

Protocol for Double-Stranded RNA Synthesis By Sudhir Nayak 1.  Make both sense and antisense RNA strands separately (we use Ambion’s RNA Transcription Kit). 2.  After the synthesis reactions, DNAse (10 units) the samples for one 30-60 min at 37 C. 3.  Mix both antisense and sense strands together in the same tube. 4.  Heat the […]

  • Tim Schedl, Ph.D
    Department of Genetics
    Campus Box #8232
    Washington University School of Medicine
    4566 Scott Ave.
    St. Louis, MO 63110


    Email: ts@genetics.wustl.edu
    PHONE: (314)362-6164 [lab]
    FAX: (314)362-7855