Schedl Lab

Washington University Genetics

Electrocompetent cells

Sudhir’s Electocompetent Cell Protocol Prepare: 200ml LB in a 1L flask 1L sterile ddH2O and place in cold room 4 50ml conicals chilled on ice 10% glycerol (cold) Chilled boxes of 100ul and 1ml pipet tips. Chilled 0.5ml tubes. General This protocol is for generating enough cells for 10-20 electoporations.  Its primary advantage is that […]

  • Tim Schedl, Ph.D
    Department of Genetics
    Campus Box #8232
    Washington University School of Medicine
    4566 Scott Ave.
    St. Louis, MO 63110

    Contact

    Email: ts@genetics.wustl.edu
    PHONE: (314)362-6164 [lab]
    FAX: (314)362-7855