Methods and Materials Cytosol extracts and immunoprecipitation Polyclonal anti-GLD-1 antibodies (Ab) have been generated (Jones et al. 1996), but they recognize the conserved GSG domain (data not shown) and therefore may disrupt interactions between GLD-1 and target RNAs. Instead, functional GLD-1 for IP was obtained from a transgenic strain in which the gld-1(q485) null mutant […]