Prepared by Sudhir Nayak The following modification to the Schedl Lab dissection and staining protocol applies to Sigma monoclonal M8159 for detecting activated MAP kinase. Staining for activated MPK-1 with Sigma monoclonal anti-activated MAP kinase (diphosphorylated ERK-1&2) antibody (M8159) in C. elegans is particularly sensitive to dissection time.  Dissection times after the addition of levamisole must […]