s.w.e Schedl Lab

Washington University Genetics

Antibody Staining of Dissected Gonads

Protocol by Ross Francis Modified by Min-Ho Lee and Sudhir Nayak 1. Dissected gonads are usually fixed formaldehyde, methanol, acetone, or some combination (see Gonad Fixation). After fixation, wash gonads 2x in PBT before starting. Using a Pasteur pipette, transfer dissected, fixed gonads to a small [6 mm (ID) x 35mm] glass culture tube (Kimax […]

  • Tim Schedl, Ph.D
    Department of Genetics
    Campus Box #8232
    Washington University School of Medicine
    4566 Scott Ave.
    St. Louis, MO 63110


    Email: ts@genetics.wustl.edu
    PHONE: (314)362-6164 [lab]
    FAX: (314)362-7855