Protocol by Ross Francis Modified by Min-Ho Lee and Sudhir Nayak 1. Dissected gonads are usually fixed formaldehyde, methanol, acetone, or some combination (see Gonad Fixation). After fixation, wash gonads 2x in PBT before starting. Using a Pasteur pipette, transfer dissected, fixed gonads to a small [6 mm (ID) x 35mm] glass culture tube (Kimax […]