Protocol by Ross Francis Modified by Sudhir Nayak To visualize DNA, incubate fixed samples with 100 ng/ml 4′,6′-diamidino-2-phenylindole hydrochloride (DAPI) in PBT. The staining time depends on the type of sample and varies from 5-30 min. For example, 5 min is long enough for dissected gonad arms but longer incubation (20-30 min) is required for […]