s.w.e Schedl Lab

Washington University Genetics

Gonad Fixation

Protocol by Ross Francis Modified by Min-Ho Lee and Sudhir Nayak A. Methanol Methanol fixation is quick, easy, and gives satisfactory nuclear morphology after DAPI staining. However, methanol-fixed gonads break easily and are more difficult to mount than formaldehyde-fixed gonads. Fix dissected gonads in 3 ml of cold (-20) methanol for 5 min. Using a […]

  • Tim Schedl, Ph.D
    Department of Genetics
    Campus Box #8232
    Washington University School of Medicine
    4566 Scott Ave.
    St. Louis, MO 63110


    Email: ts@genetics.wustl.edu
    PHONE: (314)362-6164 [lab]
    FAX: (314)362-7855