Modified by Sudhir Nayak from unknown original 1. Put 15 – 20 adult hermaphrodites on two 10 cm NGM plates and starve until L1s accumulate.  Transfer each to 100ml of liquid culture as usual and incubate 3-4 days at 20deg with shaking. 2. Harvest solutions; cold 0.1 N NaCl, cold ddH2O, cold 60% sucrose a) […]