EdU labeling and fixation (Modified by Schedl lab from protocol provided by Sarah Crittenden) To label on plates: (from Ito and McGhee, 1987) 1. Grow an overnight of MG1693 (thy-, from E. coli stock center) 2. To 100 ml of M9, add: ~2 ml ON MG1693 5 ml 20% glucoseĀ (0.4%-1% – usually 1%) 100 […]