Prepared by Jessica Kerins from unknown original *When working with EMS (or ENU), always work in fume hood and wear lab coat, goggles, and gloves. 1.  When plates have at least 50% L4s, wash 6 plates with M9 buffer (1 mL/plate) and transfer with a glass Pasteur pipette to two 15 mL conical tubes. 2.  […]