Cytosolic Extract & Immunoprecipitation Prepared by Min-Ho Lee and Sudhir Nayak 1. Culture There are 2 ways we start worm culture: EASY (but less synchronous culture): Start 100 ml liquid culture using the standard protocol from 10-20 large plates (10cm) of nearly starved N2 (lots of starved L1 on plates).  When most of the worms […]