s.w.e Schedl Lab

Washington University Genetics

Glycine elution (affinity purification)

Elution off the coloumn with Glycine-Hcl (pH 2.3) Adapted by Sudhir Nayak from Swathi Arur original. Solutions To make 0.1 M Glycine-HCl, pH 2.3: Make 100mM Glycine, and pH with HCl to a pH of 2.3, alternatively use Glycine-HCl product.  Check pH before use. Bind antibodies to your favorite protein column. 1) Wash column with […]

  • Tim Schedl, Ph.D
    Department of Genetics
    Campus Box #8232
    Washington University School of Medicine
    4566 Scott Ave.
    St. Louis, MO 63110


    Email: ts@genetics.wustl.edu
    PHONE: (314)362-6164 [lab]
    FAX: (314)362-7855