Elution off the coloumn with Glycine-Hcl (pH 2.3) Adapted by Sudhir Nayak from Swathi Arur original. Solutions To make 0.1 M Glycine-HCl, pH 2.3: Make 100mM Glycine, and pH with HCl to a pH of 2.3, alternatively use Glycine-HCl product.  Check pH before use. Bind antibodies to your favorite protein column. 1) Wash column with […]