Protocol by Eric Lambie Modified by Swathi Arur, Min-Ho Lee and Sudhir Nayak For 2 liters 1) Make NGM plates as usual 2) Autoclave 30 minutes 3) Let cool to ~47 C  in water bath Add: 2 ml 1 M CaCl2 2 ml 1 M MgSO4 2 ml 5 mg/ml cholesterol (in 95% EtOH; bring […]