Schedl Lab

Washington University Genetics

RNAi plates with lactose (instead of IPTG)

Protocol by Eric Lambie Modified by Swathi Arur, Min-Ho Lee and Sudhir Nayak For 2 liters 1) Make NGM plates as usual 2) Autoclave 30 minutes 3) Let cool to ~47 C  in water bath Add: 2 ml 1 M CaCl2 2 ml 1 M MgSO4 2 ml 5 mg/ml cholesterol (in 95% EtOH; bring […]

  • Tim Schedl, Ph.D
    Department of Genetics
    Campus Box #8232
    Washington University School of Medicine
    4566 Scott Ave.
    St. Louis, MO 63110

    Contact

    Email: ts@genetics.wustl.edu
    PHONE: (314)362-6164 [lab]
    FAX: (314)362-7855