Sudhir’s Electocompetent Cell Protocol Prepare: 200ml LB in a 1L flask 1L sterile ddH2O and place in cold room 4 50ml conicals chilled on ice 10% glycerol (cold) Chilled boxes of 100ul and 1ml pipet tips. Chilled 0.5ml tubes. General This protocol is for generating enough cells for 10-20 electoporations.  Its primary advantage is that […]