Schedl Lab

Washington University Genetics

Heat shock transformation (DH10B homemade)

Heat Shock Transformations (DH10B) Prepared by Ziva and adapted by Maia Dorsett Prior to getting cells: 1) Turn on 42 deg bath.  Takes about 30 min to reach 42 deg. 2) Put 0.1 M sterile CaCl2 on ice. 3) One tube of cells is good for several transformations. For two transformations: 1) Put 10 ul […]

  • Tim Schedl, Ph.D
    Department of Genetics
    Campus Box #8232
    Washington University School of Medicine
    4566 Scott Ave.
    St. Louis, MO 63110


    PHONE: (314)362-6164 [lab]
    FAX: (314)362-7855