Heat Shock Transformations (DH10B) Prepared by Ziva and adapted by Maia Dorsett Prior to getting cells: 1) Turn on 42 deg bath.  Takes about 30 min to reach 42 deg. 2) Put 0.1 M sterile CaCl2 on ice. 3) One tube of cells is good for several transformations. For two transformations: 1) Put 10 ul […]